Journal: Oncotarget

Article Title: Long non-coding RNA00364 represses hepatocellular carcinoma cell proliferation via modulating p-STAT3-IFIT2 signaling axis

doi: 10.18632/oncotarget.22039

Figure Lengend Snippet: (A, B) The protein level of STAT3, pSTAT3 (Tyr705), STAT1, pSTAT1 (Lys310), AKT and pAKT with LncRNA00364 overexpression or deficiency were analyzed by western blot assays. (C) Diagram showing the conserved STAT3 transcriptional response element of LncRNA00364. (D) RNA pull-down analysis to determine the interaction of phosphorylated STAT3 with mutated LncRNA00364 in HepG2 cells. (E) The distribution of pSTAT3 and STAT3 in cytoplasm and nucleus in HCC cells transfected with LncRNA00364 overexpression or vector by nucleocytoplasmic separation.

Article Snippet: Antibodies used in these assays are as follows: pSTAT1 (Lys310) (#12629), STAT1 (4135), STAT3(#4904), pSTAT3(Tyr705) (#9145), pSTAT3(Ser727) (#9134), antibodies were purchased from Cell signaling technology.

Techniques: Over Expression, Western Blot, Transfection, Plasmid Preparation

Journal: Cells

Article Title: A Novel Monoclonal Antibody Targeting Cancer-Specific Plectin Has Potent Antitumor Activity in Ovarian Cancer

doi: 10.3390/cells10092218

Figure Lengend Snippet: 1H11 induces ROS production and modulates the expression of critical proliferative and migratory effector proteins. ( A ) After 72 h of treatment, ROS levels were evaluated by DCFDA staining. 1H11 caused an increase in ROS levels. ( B , C ) Serum-starved cells were treated for 10 min, and lysates were accessed by Western blotting. 1H11 treated cells showed decreased phosphorylation of ( B ) JAK2 and ( C ) STAT3. ( D , E ) Cells were incubated with 1H11 or IgG for 72 h prior to harvest and evaluation by Western blotting. ( D ) 1H11 treatment resulted in an increased expression of two G1-checkpoint CDK inhibitors, p27 and p21. ( E ) An increase in E-cadherin and a decrease in vimentin protein expression were also observed. For Western blotting, GAPDH was used as a loading control. All experiments were performed by treating OVCAR8 cells with 125 nM 1H11 or IgG. All data plotted as mean ± SEM. *** p < 0.001, ** p < 0.01 vs. IgG. ( F ) Proposed molecular mechanism of 1H11.

Article Snippet: Primary antibodies used include pJAK2 Tyr1007/1008 (#3771), pSTAT3 Ser727 (#9134), p21 (#2947), p27 (#3686) and GAPDH (#2118) from Cell Signaling Technology (Danvers, MA, USA) as well as JAK2 (sc-390539), STAT3 (sc-8019), E-cadherin (sc-8426) and GAPDH (sc-32233) from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Staining, Western Blot, Phospho-proteomics, Incubation, Control